Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of obesity. It is known that obesity is associated with many chronic disorders such as nonalcoholic fatty liver disease nafld. An obesityassociated gut microbiome with increased capacity for energy harvest. Dietinduced obesity in zebrafish shares common pathophysiological pathways with mammalian obesity article pdf available in bmc physiology 101. Sucrose nonfermentingrelated kinase regulates both adipose. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Can a high fat paleo diet cause obesity and diabetes. We optimized protocols for generating dietinduced obese dio zebrafish model by feeding high cholesterol diet hcd. Reactive oxygen species and oxidative stress in obesity. Mar 21, 2017 different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. Dietinduced obesity in zebrafish shares common pathophysiological pathways. Obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. Childhood obesity and the zebrafish as a model for the. The aim of this study is to examine the agerelated influence of hfdinduced obesity on mandibular bone architecture and.
They are very dependent on water and you will seldom see them more than 12 km from it. The gastrointestinal gi microbiota is the collection of microbes which reside in the gi tract and represents the largest source of nonself antigens in the human body. Everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. Pdf dietinduced obesity in zebrafish shares common. Gte decreased visceral adipose tissue vat volume induced by highfat diet in zebrafish larvae. Less than 5% of chemicals were eluted from gluten granules. In this paper, hyperglycemic zebrafish, when immersed in a 111 mm glucose solution for 14 days, developed increased glycation of proteins. Different models of dietinduced obesity in zebrafish provided evidence that the pathophysiological pathways underlying dietinduced obesity in zebrafish are comparable to that of mammals, 14. The pathogenesis of nonalcoholic fatty liver disease nafld is not fully understood, and experimental models are an alternative to study this issue. Dec 19, 2011 effects of yuzu citrus junos siebold ex tanaka peel on the diet induced obesity in a zebrafish model journal of functional foods, vol. Effects of yuzu citrus junos siebold ex tanaka peel on.
International database resource for the laboratory mouse, providing integrated genetic, genomic, and biological data. It is intended to mimic the most common cause of obesity in humans. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Adult danio rerio zebrafish represents a powerful model to study both metabolic diseases and bone metabolism. Body fat accumulation in zebrafish is induced by a diet. Dietinduced dysbiosis of the intestinal microbiota and. All materials tested exhibited anti obesity effects. All materials tested exhibited antiobesity effects. The jackson laboratory maintains an inventory of diet induced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Novel antiobesity properties of palmaria mollis in zebrafish.
Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer. To evaluate the capacity of gluten to retain chemicals, we prepared gluten granules containing eight types of chemicals with different log p ow values and immersed them in water. Metabolic and bone effects of highfat diet in adult zebrafish. These diets were based the ain93g diet and beef tallow was added to provide a highfat content. Sucrose nonfermentingrelated kinase snrk is a member of the ampkrelated kinase family, and its physiological role in adipose energy homeostasis and inflammation remains unknown. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 % and 11 %, respectively. In this study, it was purposed to examine histological effects of fatty diet induced obesity on liver from female obese rats.
The purpose of this study was to establish a zebrafish model of dietinduced obesity dio. Related publications genetic determinants of energy expenditure and insulin resistance in dietinduced obesity in mice. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Future studies will enable us to understand the composite nature of mitochondrial flash activity and will encourage the generation of new indicators to better measure these phenomena.
The purpose of this study was to establish a zebrafish model of diet induced obesity dio. We carried out physiological tests, plasma lipid assays, obesity measures, liver. Jun 24, 2012 backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. Fatrich diets not only induce obesity in humans but also make. The relationship between highfat food consumption and obesity is wellestablished. Research article open access dietinduced obesity in. We have characterised molecular adaptation to high fat diet hfd feeding in inbred mouse strains widely used in genetic and physiological studies. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. We generated phf2 floxed mice, systemic phf2 null mice by crossing phf2 floxed mice with cmvcre transgenic mice, and tamoxifen. A novel protocol using gluten as a carrier material was developed to administer chemicals to adult zebrafish, per os p. The gut microbiota as an environmental factor that regulates fat storage. Compositions of real and mimetic cocoa are listed in table 2. Zebrafish is emerging as an important model for studying obesity and related. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of phagocytosis of.
Short chain fatty acids prevent highfatdietinduced obesity. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with diet induced obesity dio were evaluated. These cells were first recognized by elia metchnikoff in 1882 in the larvae of starfish upon insertion of thorns of tangerine tree and later in daphnia magna or common water flea infected with fungal spores as cells responsible for the process of. In this study, we found that pacific dulse, palmaria mollis pm, showed anti obesity effects in diet induced obesity models. Thus, zebrafish are a useful model of human dietinduced obesity, which. Zeigler zebrafish diet, 1lb bag during this covid19 pandemic, pentair global operations and supply teams are working diligently to help ensure our valued customers are getting the best possible service and delivery during this time. We investigated the effects of a simple carbohydraterich diet on the development of obesity related nafld and the impact of physical training on the metabolic abnormalities associated with this disorder. Hf or hfc diets exposure results in steatosis and hepatitis in adult fish and rodents models 25, 35.
We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes. This model has lead to many discoveries of the important signalings in obesity, such as akt and mtor. There were some differences between zebrafish and mice in how pm exerts anti obesity mechanisms. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity. Proofofconcept using pharmacokinetics and pharmacological evaluation of carbamazepine. Epigenetic regulation of adipogenesis by phf2 histone. The organs that show signs of adiposity, such as fat accumulation in the liver and visceral adipose tissues, are similar to those found in humans. Zebrafish possess many structural and functional similarities with humans and have been used to model various human diseases, including a genetic model of. The first diet induced obese dio zebrafish model was reported in 2010 oka et al.
Propensity to highfat dietinduced obesity in rats is. In this study, we found that pacific dulse, palmaria mollis pm, showed antiobesity effects in dietinduced obesity models. Hyperglycemia is the main feature for the diagnosis of this disease. The zebrafish danio rerio is an established model organism for the study of various metabolic diseases. Effects of glucose treatment up to 14 days on blood glucose levels. Although zebrafish larvae have been used in genetic and chemical screening experiments to identify novel genes involved in the regulation of energy homeostasis and potential therapeutic targets to treat obesity,23, it remains unclear whether zebrafish can be used as a model for diet induced obesity dio, similar to that observed in mammals. Fatrich diets not only induce obesity in humans but also make animals obese. Animal models of obesity are required to help us understand the signaling pathways underlying this condition. We developed a rat model of diet induced obesity that will be a powerful tool for assessment of this issue. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. Nutrigenomics of high fat diet induced obesity in mice. E2f8 promotes hepatic steatosis through fabp3 expression in. To determine whether this also occurs in larvae, zebrafish fed with hf diet and hfc diet were stained with oil red o.
The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. These data show that hyperglycemia can be induced and maintained in zebrafish. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. In this study, we used a dietinduced obesity approach in zebrafish danio. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Backhed f, ding h, wang t, hooper lv, koh gy, nagy a, et al. Short chain fatty acids prevent highfatdietinduced obesity in mice by regulating g proteincoupled receptors and gut microbiota.
Novel antiobesity properties of palmaria mollis in. A particularly interesting aspect of obesity is the differences observed in response to the. E2f8 promotes hepatic steatosis through fabp3 expression. However, it is as yet unclear whether highfat diet hfdinduced obesity in childhood and adolescence determines agerelated changes in jaw bone health. Highfat diet impairs cognitive function of zebrafish scientific reports. Dietinduced obesity in c57bl6j mice causes increased renal. Jun 19, 2007 everyone knows that eating lean fish helps slim waistlines, but researchers have found a new way fish can help eliminate obesity. Fatty diet is a significant factor causing obesity. Basic nutritional investigation ingested cocoa can prevent. Dietinduced obesity in male c57bl6 mice decreases fertility. This was an extremely high fat diet for mice, enough to render them obese and or diabetic in 4 weeks. Adult zebrafish have also been used as an obesity model. Dietinduced adipocyte number increase in adult rats. Diet induced obese dio mouse models c57bl6ntac male.
International database resource for the laboratory mouse, providing integrated. The present study was performed to investigate the molecular mechanism of 6gingerol on adipocytemediated systemic inflammation in vitro and in highfat dietinduced obese zebra fish. Rat models of dietinduced obesity and high fatlow dose. The overall gene expression profile of visceral adipose tissue is also consistent with those of humans and rodents. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes in adipose tissue in obesity. Oct 21, 2010 obesity is a multifactorial disorder influenced by genetic and environmental factors. May 20, 2015 in light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by diet induced obesity dio 15, 16, atherosclerosis induced by highcholesterol diet and in a.
High fat plus high cholesterol diet lead to hepatic. Controlled highfat diet induces an obese syndrome in rats. In four experiments, longevans rats ate ad libitum a synthetic semipurified diet containing 20 g hf or 4 g lowfat lf of fat100 g of diet or a nonpurified diet. Yuzu peel significantly suppressed the rise in plasma triacylglycerol tg and liver lipid accumulation. The burchells zebra prefer open woodland, scrub and grassland. The first dietinduced obese zebrafish model dio was reported in 2010 through. Diet induced obese dio mouse models c57bl6ntac male mice. We carried out physiological tests, plasma lipid assays, obesity. We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity. Our results suggest that a diet rich in fat, instead of protein or carbohydrate, induced body fat accumulation in zebrafish with mechanisms that might be similar to those in mammals. Lipidomic profiling of liver tissue from obesityprone and.
Effects of yuzu citrus junos siebold ex tanaka peel on the. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear because of reported. Diabetes mellitus dm affects over 10% of the worlds population. The diet contained 72% fat corn oil and lard, 28% protein, and 1% carbohydrate as energy content 17. We previously reported that snrk is ubiquitously and abundantly expressed in both white adipose tissue wat and brown adipose tissue bat, but snrk expression diminishes. Rats were examined for effects of cocoa ingestion with highfat diets. The cape mountain specie prefers the mountainous areas of the eastern and western cape in southern africa. Rnaseq based transcriptome analysis of the antiobesity effect of. Adult zebrafish were allocated to four diet groups and over 6 weeks.
Since rats and mice show a similar relationship, they are considered an appropriate model. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. There were some differences between zebrafish and mice in how pm exerts antiobesity mechanisms. In this study, we examined the in vivo role of phf2 in mice. One group of zebrafish was overfed with artemia 60 mg dry weightday fish, a living prey consisting of a relatively high amount of fat. Diet induced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Short chain fatty acids prevent highfatdietinduced. Most of those studies compared lean and obese animals and were based on excessive overfeeding of adult zebrafish with their natural diet in. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical the zebrafish is an important and widely used vertebrate model organism in scientific. The effect of diet and exercise on intestinal integrity and microbial diversity in mice. However, it is as yet unclear whether highfat diet hfd induced obesity in childhood and adolescence determines agerelated changes in jaw bone health. Persistent impaired glucose metabolism in a zebrafish. The zone diet has been consistently demonstrated in controlled clinical.
Phf2 is a jmjc family histone demethylase that removes the methyl group from h3k9me2 and works as a coactivator for several metabolismrelated transcription factors. Zebrafish as an experimental model for the study of obesity. Obesity is a multifactorial disorder influenced by genetic and environmental factors. Overfed diet induced obesity dio zebrafish exhibit increased body weight gain, hypertriglyceridemia, hepatic steatosis 23,24, and visceral adiposity 25,26. Body fat accumulation in zebrafish is induced by a diet rich. We investigated the effects of a simple carbohydraterich diet on the development of obesityrelated nafld and the impact of physical training on the metabolic abnormalities associated with this disorder. Nov 23, 2015 obesity is a multifactorial health problem resulting from genetic, environmental and behavioral factors. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. Backhed f, manchester jk, semenkovich cf, gordon ji 2007 mechanisms underlying the resistance to dietinduced obesity in germfree mice. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Consequently, zebrafish might serve as a good animal model for. Feeding hcd for 8 weeks resulted in exogenous obesity confirmed by higher body mass index bmi values of obese males 0. Hf and hfc dietsinduced hepatic steatosis in zebrafish larvae. High fat plus high cholesterol diet lead to hepatic steatosis.
The aim of this study is to examine the agerelated influence of hfd induced obesity on mandibular bone architecture and the structure of the periodontium in. A novel protocol for the oral administration of test. Researchers now describe the first genetic model of obesity in a fish. Turnbaugh pj, ley re, mahowald ma, magrini v, mardis er, gordon ji.
Moreover, a useful zebrafish model of diet induced obesity dio has been created and was found to be highly consistent with the obesity observed in humans and in rodent models of dio oka et al. Highfat dietinduced obesity triggers alveolar bone loss and. The gi tract functions as a major immunological organ as it must maintain tolerance to commensal and dietary antigens while remaining responsive to pathogenic stimuli. Nutritional factors play important roles in the etiology of obesity, type 2 diabetes mellitus and their complications through genotype x environment interactions. Dec 22, 2017 an increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Effects of yuzu peel citrus junos siebold ex tanaka, yuzu pomace after hexane extraction, and auraptene on metabolic disorders in zebrafish with dietinduced obesity dio were evaluated. Macrophages are ubiquitously present innate immune cells in humans and animals belonging to both invertebrates and vertebrates. Dietinduced obesity in c57bl6j mice causes increased. A novel wistar rat model of obesityrelated nonalcoholic. Research article a novel wistar rat model of obesity.
Thus, zebrafish are a useful model of human diet induced obesity 11 14, which led us to hypothesize that if a hf diet induces cognitive dysfunction in zebrafish, then zebrafish could also be a simple model for the screening of compounds with activity against dementia induced by unhealthy diet or obesity. The aim of this study was to generate an obese adult zebrafish. Anti obesity mechanisms of pm in zebrafish and mice models of obesity. Barry sears, to reduce the levels of dietinduced inflammation. Antiobesity effects of boiled tuna extract in mice with. Dietinduced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome. In light of these similarities to mammals and the advantages of the system, the zebrafish model has been used in the field of lipid metabolism research for studies of lipidrelated diseases, including dyslipidaemia with hepatic steatosis induced by dietinduced obesity dio 15, 16, atherosclerosisinduced by highcholesterol diet and in a. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Larvae fed with control diet rarely developed steatosis after 7 and 10 days feeding 0 %. Nov 29, 2012 carbohydratefree diet for 2 or 4 weeks following protocols. Highfat dietinduced obesity triggers alveolar bone loss. Hf and hfc diets induced hepatic steatosis in zebrafish larvae.
The dietinduced obesity model closely mimics the increasingly availability of the highfathighdensity foods in modern society over the past two decades, which are main contributors to the obesity trend in human. Although the zebrafish is a model that has become popular in recent decades for basic biomedical research due to the multiple advantages highlighted in several articles 40,41, it has not been used extensively for studies related to obesity and diet. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. The zone diet is a nutritional program developed by dr. Highfat diet impairs cognitive function of zebrafish. Sucrose nonfermentingrelated kinase regulates both. Overfeedinginduced obesity in zebrafish shows similar symptoms to those in.
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